BBa_J23016
BBa_J23016 Version 1 (Component)
F plasmid with trbC knockout, CmR (graveyarded)

BBa_J23057
BBa_J23057 Version 1 (Component)
TraGr of Rlambda, marker excised (Graveyarded)

BBa_J23067
BBa_J23067 Version 1 (Component)
Intermediate in construction of TraGr cassette

BBa_J23068
BBa_J23068 Version 1 (Component)
Intermediate #2 in construction of TraGr cassette

BBa_J23056
BBa_J23056 Version 1 (Component)
pOX38/MC1061 trbCf knockout, marker excised (Graveyarded)

BBa_J120014
BBa_J120014 Version 1 (Component)
Binding sites for Pax3 and Sox10 with GFP selectable marker and double terminator

BBa_K1778006
BBa_K1778006 Version 1 (Component)
Kana-His is a selective marker gene in experiment.

BBa_J23017
BBa_J23017 Version 1 (Component)
RLambda traG knockout (graveyarded)

BBa_M1654
BBa_M1654 Version 1 (Component)
S100 calcium biomarker

BBa_M1659
BBa_M1659 Version 1 (Component)
Composite part for lung cancer biomarker

BBa_K1020005
BBa_K1020005 Version 1 (Component)
Alkane Marker--Device for detection of various alkanes

BBa_K1207001
BBa_K1207001 Version 1 (Component)
Macrolide-Lincosamide-Streptogramin B Resistance Marker

BBa_K1486031
BBa_K1486031 Version 1 (Component)
CaUra3 selection marker

KanMX(lox)
BBa_K300989 Version 1 (Component)
[LoxP-KanMX-LoxP] excisable marker for yeast

BBa_K322922
BBa_K322922 Version 1 (Component)
cat + sacB for BRIDGE: A biobrick compatible markerless recombineering method

BBa_K1351023
BBa_K1351023 Version 1 (Component)
upp cassette as counter-selection marker

mRFP sel.
BBa_K1362422 Version 1 (Component)
mRFP selection marker with outward BsaI restriction sites

BBa_K322710
BBa_K322710 Version 1 (Component)
Marker construct which can be used to target tnaA in E.coli.

argB
BBa_K678043 Version 1 (Component)
argB cassette, selection marker for Aspergillus nidulans

bleR
BBa_K678044 Version 1 (Component)
bleR cassette, selection marker for Aspergillus nidulans

ptrA
BBa_K678045 Version 1 (Component)
ptrA, selection marker for Aspergillus nidulans

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K120016
BBa_K120016 Version 1 (Component)
Gal1-His3_marker

BBa_K272997
BBa_K272997 Version 1 (Component)
Rbs-Spectinomycin resistance marker-terminator

BBa_K272500
BBa_K272500 Version 1 (Component)
Constitutive expression cassette for TetR repressor-mRFP-Spectinomycin resistance marker

BBa_K272994
BBa_K272994 Version 1 (Component)
Rbs-Streptomycin resistance marker-terminator

BBa_K272992
BBa_K272992 Version 1 (Component)
Rbs-Streptomycin resistance marker-ECFP

BBa_K272991
BBa_K272991 Version 1 (Component)
Rbs-ccdB toxin - EYFP-constitutive promoter-Rbs-Streptomycin resistance marker-ECFP